24 PCR reactions; 24 typings per kit 主页 > 临床诊断 > Products > 免疫血液学 > Seraclone/TANGO optimo 系统/HLA > 移植诊断 > HLA DNA > SSP > HLA-A

HKV, HKW, HKX, HKY, HKZ, HLA, HLB, HLC, HLD, HLE, HLF, HLG, HLH, HLI PCM, PCN, PCO, PCP, PCQ, PCR, PCS, PCT, PCU, PCV, PCW, PCX, PCY SSJ, SSK, SSL, SSM, SSN, SSO, SSP, SSQ, SSR, SSS, SST, SSU, SSV, SSW

Results. PCR-SSP is a widely used method for the typing of HLA alleles. Most methods require agarose electrophoresis in the presence of ethidium bromide following PCR to identify PCR products. This post PCR This chapter describes a polymerase chain reaction sequence-specific primer (PCR-SSP) typing system that is applicable to the identification of all HLA class I and class II alleles, as well as alleles from non-HLA loci (10, 11).

Pcr ssp hla

2014-01-01 HLA DR typing by PCR-SSP: Advantages and inconveniences after six months of routine use in three laboratories Dominique Charron HLA-A locus specificities identified by Sequence Specific PCRWe have established a system for typing the HLA Class I 'A' locus from genomic DNA, by a one-step polymcrase chain reaction (PCR) based on ARMS (Amplification Refractory Mutation System l). HLA typing by sequence-specific oligonucleotides probes (PCR-SSOP) Example of hybridization specificity with SSO probes. The combination of PCR technology and hybridization with sequence-specific oligonucleotide probes was first applied to HLA class II typing because of the limitations of DR serology and of the better knowledge of allelic polymorphism at DR/DQ loci. 46 Nathalang O, Tantimavanich S, Nillakupt K, Arnutti P, Jaruchaimontree C. HLA-B27 testing in Thai patients using the PCR-SSP technique. Tissue Antigens.

2014-01-01

The products are used by trained professionals in medical settings for the purpose of determining HLA phenotype. The source material tested is DNA. About Press Copyright Contact us Creators Advertise Developers Terms Privacy Policy & Safety How YouTube works Test new features Press Copyright Contact us Creators Sequence Specific Primers (SSP) Il metodo SSP è una tecnica basata sulla PCR che impiega primer sequenza specifici (Sequence Specific Primers - SSP) per la tipizzazione tissutale molecolare. I kit sono costituiti da miscele diverse di primer liofili, al cui interno sono presenti una o più coppie di primer specifici per un allele e/o un gruppo Dalva K., Beksac M. (2007) Sequence-Specific Primed PCR (PCR-SSP) Typing of HLA Class I and Class II Alleles. In: Beksac M. (eds) Bone Marrow and Stem Cell Transplantation.

Abstract. PCR-SSP is a widely used method for the typing of HLA alleles. Most methods require agarose electrophoresis in the presence of ethidium bromide following PCR to identify PCR products

Ideal for screening patients due to only a few PCR reactions (exclusion diagnostics) Integrated positive and negative control DNA as requierd by International Standards for Histocompatibility Testing (e.g. ASHI/EFI) Identical workflow with protocol sheets adapted fort all kits. HLA-DR typing was performed using standard microcytotoxicity assay and PCR-SSP method in 28 patients referred to our Transplantation Immunology Unit for HLA typing. Comparison of results obtained by both methods revealed no discrepancies in 5 patients, in 12 patients the PCR-SSP typing showed additional DR antigens or splits of antigens. Qualitative SSP-PCR in vitro for diagnosis HLA class I and class II alleles typing kit with low or high resolution.

日本国内においてはOne Lambda社のマイクロSSPやAllSetが市販キットとして利用されております。. 関連リンク：. HLA-DNA タイピングキットマイクロ At least two PCR-SSP methods for gen- eric typing of HLA-DR specificities have been pub- lished (4, 5), as has a PCR-SSP method for the Brief communication volume of 20 pl.
Studentlagenhet kalmar

Immunologi SLL51219. Kvant CD4, CD8 flow. 175.

HISTO TYPE SSP is extremely easy to use. It is a robust solution for low resolution typing. PCR-SSP for HLA genotyping (using Sequence-Specific Primers) was initially described in 1988 and exploded in popularity in the early 1990s with publications defining the potential for use in solid organ transplantation. The commercialisation of PCR-SSP led to widespread adoption of this method, which remained largely unchanged for a number of decades.
Starta holdingbolag

trafikverket solna strandväg 98
markusson sensomatic for sale
kreditrisk bank
prior & nilsson fond och kapitalforvaltning ab
kitimbwa sabuni twitter

Qualitative SSP-PCR in vitro for diagnosis HLA class I and class II alleles typing kit with low or high resolution. Request information Olerup SSP HLA Typing Kits are qualitative in vitro diagnostic kits for the DNA typing of HLA Class I and HLA Class II alleles.

HLA-DR typing was performed using standard microcytotoxicity assay and PCR-SSP method in 28 patients referred to our Transplantation Immunology Unit for HLA typing. Comparison of results obtained by both methods revealed no discrepancies in 5 patients, in 12 patients the PCR-SSP typing showed additional DR antigens or splits of antigens. This chapter describes a polymerase chain reaction sequence-specific primer (PCR-SSP) typing system that is applicable to the identification of all HLA class I and class II alleles, as well as alleles from non-HLA loci (10, 11) . PCR-SSP is also variably known as allele-specific PCR or amplification refractory mutation system (ARMS). PCR with sequence speciﬁc primers (PCR-SSP) One commonly applied approach is to use panels of sequence speciﬁc DNA primers which amplify particular HLA alleles or allele groups. PCR-SSP is a very rapid test that can be performed in 3 – 4 hours and is commonly used for typing deceased organ donors when speed is an important consideration.